کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5544943 1555225 2016 25 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection and prevalence of boid inclusion body disease in collections of boas and pythons using immunological assays
ترجمه فارسی عنوان
تشخیص و شیوع بیماری جسمی غوطه ور در جمع آوری بو ها و پایتون ها با استفاده از آزمون های ایمونولوژیک
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
The overall prevalence of IBD in study snakes was 25/131 or 19% (95% CI = 12.4%, 25.8%) with boa constrictors being more commonly infected (22/53 or 41.5%; 95% CI = 28.2%, 54.8%) than other species in this study. Of the 22 IBD positive boa constrictors, 87% were clinically healthy, 13% had various signs of chronic illness, and none showed signs of central nervous system disease. Using a validated monoclonal anti-NP antibody, NP was confirmed within the isolated PWBC by immunohistochemical staining and Western blots. The presence of reptarenaviruses within blood samples of 27 boa constrictors and three rainbow boas was also assessed by PCR. Among boa constrictors, very good agreements were shown between the observation of inclusion bodies (by HE stain) and the presence of NP (by immunohistochemistry, kappa = 0.92; and Western blots, kappa = 0.89), or the presence of reptarenaviruses (by PCR; kappa = 0.92).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 218, December 2016, Pages 13-18
نویسندگان
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