کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5544963 | 1555226 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Canine mesenchymal stem cells are neurotrophic and angiogenic: An in vitro assessment of their paracrine activity
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Canine mesenchymal stem cells are neurotrophic and angiogenic: An in vitro assessment of their paracrine activity Canine mesenchymal stem cells are neurotrophic and angiogenic: An in vitro assessment of their paracrine activity](/preview/png/5544963.png)
چکیده انگلیسی
Mesenchymal stem cells (MSCs) have been used in cell replacement therapies for connective tissue damage, but also can stimulate wound healing through paracrine activity. In order to further understand the potential use of MSCs to treat dogs with neurological disorders, this study examined the paracrine action of adipose-derived canine MSCs on neuronal and endothelial cell models. The culture-expanded MSCs exhibited a MSC phenotype according to plastic adherence, cell morphology, CD profiling and differentiation potential along mesenchymal lineages. Treating the SH-SY5Y neuronal cell line with serum-free MSC culture-conditioned medium (MSC CM) significantly increased SH-SY5Y cell proliferation (Pâ<0.01), neurite outgrowth (Pâ=â0.0055) and immunopositivity for the neuronal marker βIII-tubulin (Pâ=â0.0002). Treatment of the EA.hy926 endothelial cell line with MSC CM significantly increased the rate of wound closure in endothelial cell scratch wound assays (Pâ=â0.0409), which was associated with significantly increased endothelial cell proliferation (Pâ<0.05) and migration (Pâ=â0.0001). Furthermore, canine MSC CM induced endothelial tubule formation in EA.hy926 cells in a soluble basement membrane matrix. Hence, this study has demonstrated that adipose-derived canine MSC CM stimulated neuronal and endothelial cells probably through the paracrine activity of MSC-secreted factors. This supports the use of canine MSC transplants or their secreted products in the clinical treatment of dogs with neurological disorders and provides some insight into possible mechanisms of action.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 217, November 2016, Pages 10-17
Journal: The Veterinary Journal - Volume 217, November 2016, Pages 10-17
نویسندگان
I.R. Al Delfi, J.J. Sheard, C.R. Wood, A. Vernallis, J.F. Innes, P. Myint, W.E.B. Johnson,