کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5584963 | 1568004 | 2016 | 12 صفحه PDF | دانلود رایگان |

- Short term (1 and 3 wk) effects of DHA intake (1076 mg/d) on FA profiles.
- Long term (6 and 12 wk) effects of DHA intake on FA profiles.
- Massive increase of DHA levels in plasma and red blood cells.
- Slightly, but significantly increased EPA levels in plasma.
- Estimated %-retroconversion rate: 13.2, 12.0, 5.3, and 3.3 after 1, 3, 6, and 12Â wk.
IntroductionPolyunsaturated fatty acids (PUFA) are metabolized in a complex network of elongation, desaturation and beta oxidation.Material and methodsThe short (1 and 3 wk), and long term (6 and 12 wk) effect of 1076 mg/d docosahexaenoic acid (DHA, free of eicosapentaenoic acid (EPA)) on (absolute) PUFA concentrations in plasma and red blood cells (RBC) of 12 healthy men (mean age 25.1±1.5 years) was investigated.ResultsRBC DHA concentrations significantly (p<0.001) increased from 28±1.6 µg/mL to 38±2.0 µg/mL (wk 1), 52±3.3 µg/mL (wk 3), 68±2.6 µg/mL (wk 6), and 79±3.5 µg/mL (wk 12). Arachidonic acid (AA) concentrations declined in response to DHA treatment, while the effect was more pronounced in plasma (wk 0: 183±9.9 µg/mL, wk 12: 139±8.0 µg/mL, â24%, p<0.001) compared to RBC (wk 0: 130±3.7 µg/mL, wk 12: 108±4.0 µg/mL, â16%, p=0.001). Furthermore, an increase of EPA concentrations in plasma (wk 0: 15±1.5 µg/mL, wk 1:19±1.6 µg/mL, wk 3: 27±2.3 µg/mL, wk 6: 23±1.2 µg/mL, wk 12: 25±1.7 µg/mL, p<0.001) and RBC (wk 0: 4.7±0.33 µg/mL, wk 1: 6.7±1.3 µg/mL, wk 3: 8.0±0.66 µg/mL, wk 6: 6.9±0.44 µg/mL, wk 12: 6.7±0.45 µg/mL, n.s.) was observed suggesting a retroconversion of DHA to EPA.ConclusionBased on PUFA concentrations we showed that DHA supplementation results in increased EPA levels, whereas it is not known if this impacts the formation of EPA-derived lipid mediators. Furthermore, shifts in the entire PUFA pattern after supplementation of EPA or DHA should be taken into account when discussing differential physiological effects of EPA and DHA.
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - Volume 115, December 2016, Pages 12-23