کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5584963 1568004 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of docosahexaenoic acid supplementation on PUFA levels in red blood cells and plasma
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Effects of docosahexaenoic acid supplementation on PUFA levels in red blood cells and plasma
چکیده انگلیسی


- Short term (1 and 3 wk) effects of DHA intake (1076 mg/d) on FA profiles.
- Long term (6 and 12 wk) effects of DHA intake on FA profiles.
- Massive increase of DHA levels in plasma and red blood cells.
- Slightly, but significantly increased EPA levels in plasma.
- Estimated %-retroconversion rate: 13.2, 12.0, 5.3, and 3.3 after 1, 3, 6, and 12 wk.

IntroductionPolyunsaturated fatty acids (PUFA) are metabolized in a complex network of elongation, desaturation and beta oxidation.Material and methodsThe short (1 and 3 wk), and long term (6 and 12 wk) effect of 1076 mg/d docosahexaenoic acid (DHA, free of eicosapentaenoic acid (EPA)) on (absolute) PUFA concentrations in plasma and red blood cells (RBC) of 12 healthy men (mean age 25.1±1.5 years) was investigated.ResultsRBC DHA concentrations significantly (p<0.001) increased from 28±1.6 µg/mL to 38±2.0 µg/mL (wk 1), 52±3.3 µg/mL (wk 3), 68±2.6 µg/mL (wk 6), and 79±3.5 µg/mL (wk 12). Arachidonic acid (AA) concentrations declined in response to DHA treatment, while the effect was more pronounced in plasma (wk 0: 183±9.9 µg/mL, wk 12: 139±8.0 µg/mL, −24%, p<0.001) compared to RBC (wk 0: 130±3.7 µg/mL, wk 12: 108±4.0 µg/mL, −16%, p=0.001). Furthermore, an increase of EPA concentrations in plasma (wk 0: 15±1.5 µg/mL, wk 1:19±1.6 µg/mL, wk 3: 27±2.3 µg/mL, wk 6: 23±1.2 µg/mL, wk 12: 25±1.7 µg/mL, p<0.001) and RBC (wk 0: 4.7±0.33 µg/mL, wk 1: 6.7±1.3 µg/mL, wk 3: 8.0±0.66 µg/mL, wk 6: 6.9±0.44 µg/mL, wk 12: 6.7±0.45 µg/mL, n.s.) was observed suggesting a retroconversion of DHA to EPA.ConclusionBased on PUFA concentrations we showed that DHA supplementation results in increased EPA levels, whereas it is not known if this impacts the formation of EPA-derived lipid mediators. Furthermore, shifts in the entire PUFA pattern after supplementation of EPA or DHA should be taken into account when discussing differential physiological effects of EPA and DHA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - Volume 115, December 2016, Pages 12-23
نویسندگان
, , , , , , , ,