کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5589137 1569804 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genetic regulation of adipose tissue transcript expression is involved in modulating serum triglyceride and HDL-cholesterol
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Genetic regulation of adipose tissue transcript expression is involved in modulating serum triglyceride and HDL-cholesterol
چکیده انگلیسی


• Derangement of transcript expression in adipose can modulate serum triglyceride (TRIG) and HDL-Cholesterol (HDL-C) level.
• In adipose immuno-inflammatory pathway genes were activated among individuals with higher serum TRIG and low HDL-C level.
• HDL-C-associated transcripts were enriched for branched chain amino acid catabolism, and oxidative phosphorylation genes.
• Expressions of serum lipid-associated transcripts in adipose are dependent on cis-regulatory polymorphisms (cis-eSNPs).
• Genetically-mediated transcriptional regulation in adipose may play a role in reducing HDL-C and increasing TRIG in serum.

Dyslipidemia is a major contributor to the increased cardiovascular disease and mortality associated with obesity and type 2 diabetes. We hypothesized that variation in expression of adipose tissue transcripts is associated with serum lipid concentrations in African Americans (AAs), and common genetic variants regulate expression levels of these transcripts. Fasting serum lipid levels, genome-wide transcript expression profiles of subcutaneous adipose tissue, and genome-wide SNP genotypes were analyzed in a cohort of non-diabetic AAs (N = 250). Serum triglyceride (TRIG) and high density lipoprotein-cholesterol (HDL-C) levels were associated (FDR < 0.01) with expression level of 1021 and 1875 adipose tissue transcripts, respectively, but none associated with total cholesterol or LDL-C levels. Serum HDL-C-associated transcripts were enriched for salient biological pathways, including branched-chain amino acid degradation, and oxidative phosphorylation. Genes in immuno-inflammatory pathways were activated among individuals with higher serum TRIG levels. We identified significant cis-regulatory SNPs (cis-eSNPs) for 449 serum lipid-associated transcripts in adipose tissue. The cis-eSNPs of 12 genes were nominally associated (p < 0.001) with serum lipid level in genome wide association studies in Global Lipids Genetics Consortium (GLGC) cohorts. Allelic effect direction of cis-eSNPs on expression of MARCH2, BEST1 and TMEM258 matched with effect direction of these SNP alleles on serum TRIG or HDL-C levels in GLGC cohorts. These data suggest that expressions of serum lipid-associated transcripts in adipose tissue are dependent on common cis-eSNPs in African Americans. Thus, genetically-mediated transcriptional regulation in adipose tissue may play a role in reducing HDL-C and increasing TRIG in serum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 632, 20 October 2017, Pages 50–58