کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589160 | 1569808 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Zoantharian mitochondrial genomes contain unique complex group I introns and highly conserved intergenic regions
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کلمات کلیدی
gDNAIon Torrent PGMORFPGMMitogenomeIGRNADH dehydrogenaseHoming endonuclease geneMitochondrial DNA - DNA میتوکندریاgenomic DNA - DNA ژنومیhEG - HEGGroup I intron - اینترانت گروه ImtDNA - دیانای میتوکندریاییcytochrome oxidase - سیتوکروم اکسیدازopen reading frame - قاب خواندن بازIntergenic region - منطقه مصنوعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Complex group I introns represent hallmarks of hexacoral mitochondrial genomes (mtDNAs). These intron elements are expected to influence the gene organization and gene expression. We sequenced the mitochondrial genome and transcriptome of Zoanthus sansibariscus and Palythoa heliodiscus, two zoantharian species (colonial anemones) representing different families within the suborder Brachycnemina. The circular and approximately 21Â kb mtDNAs contained two group I introns, one in ND5 and another in COI. The ND5-717 intron harbored two conventional mitochondrial genes (ND1 and ND3) within its structure and revealed several conserved features compared to ND5-717 in sea anemones. The COI intron, however, was inserted at a unique location (after position 867), which was different from that in sea anemones (position 884) and stony corals (position 720). COI-867 contained a homing endonuclease gene (HEG) with remarkable features, including species-specific length variations and only one copy of the essential LAGLIDADG motif. Whereas transcriptome analysis indicated that all conventional mtDNA genes were expressed, HEG expression appeared significantly repressed. Finally, we identified absolutely conserved non-coding repeat motifs with antisense features and potential regulatory functions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 628, 10 September 2017, Pages 24-31
Journal: Gene - Volume 628, 10 September 2017, Pages 24-31
نویسندگان
Sylvia Ighem Chi, Steinar D. Johansen,