کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589250 | 1569810 | 2017 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression pattern and functional analysis of fundc1 in rare minnow (Gobiocypris rarus)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ORFBNIP3LCANXFUNDC1LC3BCK2BNIP3MAMNCBILIR - بهrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدEmbryogenesis - جنین زاییtransmembrane - فرابنفشopen reading frame - قاب خواندن بازNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیRace - مسابقهLC3-interacting region - منطقه LC3 تعاملUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهRare minnow - ناهنجاری نادرHypoxia - هیپوکسیmicrotubule-associated protein 1 light chain 3 beta - پروتئین مرتبط با میکروتوبول 1 زنجیره سبک 3 بتاCasein kinase 2 - کازئین کیناز 2calnexin - کلنکسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression pattern and functional analysis of fundc1 in rare minnow (Gobiocypris rarus) Expression pattern and functional analysis of fundc1 in rare minnow (Gobiocypris rarus)](/preview/png/5589250.png)
چکیده انگلیسی
Fundc1 is a mitochondrial outer membrane protein and plays important roles in mitochondria fission and hypoxia-induced mitophagy in mammalian cells. However, there is no relevant report of fundc1 in fish. In the present study, we cloned a 942Â bp fundc1 cDNA from rare minnow. The cDNA, designated as Grfundc1 cDNA, contains an open reading frame (ORF) of 459Â bp which encodes a polypeptide of 152 amino acid residues. Comparisons of deduced amino acid sequences demonstrated that Grfundc1 was highly homologous with those of other vertebrates. RT-PCR and real time PCR detection revealed that the transcripts of Grfundc1 were not detectable in the unfertilized eggs and had high levels at blastula and gastrula stages. Whole mount in situ hybridization analysis observed that Grfundc1 was ubiquitously expressed at early stage and later riched in specific regions, such as brain, branchial arch, eye and somite during embryogenesis. Grfundc1 was expressed in all the tissues of rare minnow adult, including brain, liver, gill, eyes, heart, kidney, intestine, muscle, testis and ovary. The expression of Grfundc1 in the brain, gill, heart and eye of rare minnow adult was significantly down-regulated by hypoxia. Similar hypoxic response was observed in the rare minnow embryos at 48Â hpf following hypoxia exposure. Functional analysis showed that knockdown of Grfundc1 significantly caused defects in the body axis and dorsal neural tissues of rare minnow embryos. These results indicate that Grfundc1 may play important roles in embryogenesis in fish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 626, 30 August 2017, Pages 149-157
Journal: Gene - Volume 626, 30 August 2017, Pages 149-157
نویسندگان
Gongyu Xu, Zhenzhen Li, Jinwen Xiao, Fangqing Li, Weiyuan Ye, Haobin Zhao, Qingchun Zhou, Xueping Zhong,