کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5589267 1569810 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperIdentification of the conserved and novel microRNAs by deep sequencing and prediction of their targets in Topmouth culter
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Research paperIdentification of the conserved and novel microRNAs by deep sequencing and prediction of their targets in Topmouth culter
چکیده انگلیسی


- A total of 273 conserved and 8 novel miRNAs were identified in topmouth culter by deep sequencing approach firstly.
- 8 novel identified miRNAs expression profile of topmouth culter were determined by Stem-loop qRT-PCR.
- 1 413 targets were predicted and these target genes were analysis by GO and KEGG.

MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play important roles in regulation of almost all biological processes in organisms. Topmouth culter is an economically important fish species in the Chinese freshwater polyculture system for its delicacy and high economic value. However, to date, no miRNAs have been reported in Topmouth culter species. In this study, the liver, muscle, spleen and kidney of Topmouth culter are sampled and used for construction of a small RNA library for deep sequencing. A total of 273 conserved miRNAs/miRNAs-star and 8 novel miRNAs/miRNAs-star were discovered, these miRNAs belong to 86 different miRNA families. There is a strong base bias on miRNA nucleotide compositions at certain positions. U is the dominant nucleotide, particularly at the beginning, middle, and end of conserved miRNAs. The expression of all 8 novel identified miRNAs in Topmouth culter was validated by using stem-loop qRT-PCR. Furthermore, the potential targets were predicted for these miRNAs, which were found to be involved in diverse biological processes. Our study is the first significantly enriches the Topmouth culter miRNA repertoire and provides a reference for further elucidation of complex miRNA-mediated regulatory networks for gene expression in the growth and developmental progression of Topmouth culter.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 626, 30 August 2017, Pages 298-304
نویسندگان
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