کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589279 | 1569810 | 2017 | 51 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptome profiling of the floral buds and discovery of genes related to sex-differentiation in the dioecious cucurbit Coccinia grandis (L.) Voigt
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کلمات کلیدی
SnRK2GH3TIR1Coccinia grandisbHLHBRI1qRT-PCRSUFEIN3HD-ZIPBSKRNA-seqCoGAGLACSSPF1-aminocyclopropane-1-carboxylate synthase - 1-آمینوسیلیکوپروپان-1-کربوکسیلات سنتازZinc finger - انگشت رویBak1 - بک 1RNA sequencing - ترتیب RNASex determination - تعیین جنسیتGenome annotation - حاشیه نویسی ژنومKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوDioecious - دیوئهquantitative reverse transcription PCR - رونویسی معکوس PCR کمیSAUR - ساورDEG - شماTranscription factors - عوامل رونویسیauxin response factors - عوامل پاسخ اکسینMyb - مبینArfs - وراثتTransport inhibitor response 1 - پاسخ مهار کننده حمل و نقل 1Differentially expressed genes - ژن های متفاوت بیان شده است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dioecious species offer an inclusive structure to study the molecular basis of sexual dimorphism in angiosperms. Despite having a small genome and heteromorphic sex chromosomes, Coccinia grandis is a highly neglected dioecious species with little information available on its physical state, genetic orientation and key sex-defining elements. In the present study, we performed RNA-Seq and DGE analysis of male (MB) and female (FB) buds in C. grandis to gain insights into the molecular basis of sex determination in this plant. De novo assembly of 75 million clean reads resulted in 72,479 unigenes for male library and 63,308 unigenes for female library with a mean length of 736Â bp. 61,458 (85.57%) unigenes displayed significant similarity with protein sequences from publicly available databases. Comparative transcriptome analyses revealed 1410 unigenes as differentially expressed (DEGs) between MB and FB samples. A consistent correlation between the expression levels of DEGs was observed for the RNA-Seq pattern and qRT-PCR validation. Functional annotation showed high enrichment of DEGs involved in phytohormone biosynthesis, hormone signaling and transduction, transcriptional regulation and methyltransferase activity. High induction of hormone responsive genes such as ARF6, ACC synthase1, SNRK2 and BRI1-associated receptor kinase 1 (BAK1) suggest that multiple phytohormones and their signaling crosstalk play crucial role in sex determination in this species. Beside, the transcription factors such as zinc fingers, homeodomain leucine zippers and MYBs were identified as major determinants of male specific expression. Moreover, the detection of multiple DEGs as the miRNA target site implies that a small RNA mediated gene silencing cascade may also be regulating gender differentiation in C. grandis. Overall, the present transcriptome resources provide us a large number of DEGs involved in sex expression and could form the groundwork for unravelling the molecular mechanism of sex determination in C. grandis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 626, 30 August 2017, Pages 395-406
Journal: Gene - Volume 626, 30 August 2017, Pages 395-406
نویسندگان
Jatindra Nath Mohanty, Sanghamitra Nayak, Sumita Jha, Raj Kumar Joshi,