Chronic endosulfan exposure impairs immune response rendering Clarias gariepinus susceptible to microbial infection

Endosulfan, a persistent organochlorine insecticide affects several off-target organisms including fish though the underlying mechanisms remain obscure. In the present study, we monitored the effect of chronic endosulfan exposure on headkidney (HK), an important immune organ in fish and on fish immune system thereof. Clarias gariepinus were exposed to a non-lethal concentration of endosulfan 2.884 ppb (1/10th LC50) for 30 d which resulted in suppressed phagocytosis and bactericidal potential of headkidney macrophages (HKM). The same non-lethal concentration of endosulfan also interfered with T-cell proliferation and serum antibody titer in fish. Endosulfan-exposed fish were challenged with non-lethal dose of fish pathogenic bacteria Aeromonas hydrophila and the 'exposure-challenge' study revealed endosulfan-exposed C. gariepinus severely immunocompromised and prone to bacterial infections. Depuration for 30 d suggested that except for phagocytosis and serum agglutination titer other endosulfan-induced immune aberrations could not be restored significantly. Nonetheless, compared to exposed-challenged fish the depurated fish showed significant improvement in viability on challenge with A. hydrophila. Collectively, these findings suggest chronic endosulfan exposure has prolonged effect on fish making them prone to microbial infections.