کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5765615 | 1626779 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
On the way for detecting and quantifying elusive species in the sea: The Octopus vulgaris case study
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Environmental DNA (eDNA) can be a powerful method for assessing the presence and the distribution of aquatic species. We used this tool in order to detect and quantify eDNA from the elusive species Octopus vulgaris, using qPCRs (SybrGreen protocol). We designed species-specific primers, and set up an experimental aquarium approach to validate the new molecular tool in different controlled conditions. Field validation was conducted from sea water samples taken from 8 locations within an octopus fishery area in the Cantabrian Sea during February-March 2016. A significant positive correlation between the total biomass (g of O. vulgaris within thanks) and the amount of O. vulgaris eDNA detected (p-value = 0.01261) was found in aquarium experiments. The species was also detected by PCR in 7 of the 8 water samples taken at sea, and successfully quantified by qPCR in 5 samples. This preliminary study and innovative method opens very promising perspectives for developing quick and cheap tools for the assessment of O. vulgaris distribution and abundance in the sea. The method could help in a close future for quantifying unseen and elusive marine species, thus contributing to establish sustainable fisheries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fisheries Research - Volume 191, July 2017, Pages 41-48
Journal: Fisheries Research - Volume 191, July 2017, Pages 41-48
نویسندگان
Q. Mauvisseau, M. Parrondo, M.P. Fernández, L. GarcÃa, J.L. MartÃnez, E. GarcÃa-Vázquez, Y.J. Borrell,