کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5796673 | 1555016 | 2015 | 5 صفحه PDF | دانلود رایگان |
- MAbs against goose CD3É extracellular domain was produced and characterized for the first time.
- MAb 3C11 and 5A3 had excellent immunoreactivity with rGoCD3Éex, peripheral blood lymphocytes and the polypeptide of goose CD3É expressed in the eukaryotic cells.
- MAb 3C11 and 5A3 could be used to detect the percentage and shape of CD3+ T lymphocytes.
CD3 is one of the most important cell surface markers of T lymphocytes, and has an important function in signal transmission during antigen recognition. In this study, monoclonal antibodies against goose CD3É extracellular domain were developed and characterized for the first time. The purified recombinant goose CD3É extracellular domain protein and pcDNA3.1-GoCD3Éex were used as immunogens to develop mAbs. Furthermore, recombinant protein was used to screen for mAbs. The characteristics of mAbs were identified through IFA, I-ELISA, Western blot, FCM, and LSCM analyzes. Results showed that mAbs 3C11 and 5A3 may be good candidates for use in detecting goose T lymphocytes and in immunoassaying and exploring the function of the CD3É molecule.
Journal: Veterinary Immunology and Immunopathology - Volume 168, Issues 3â4, 15 December 2015, Pages 135-139