کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5796753 | 1555025 | 2015 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Immune gene expression in the spleen of chickens experimentally infected with Ascaridia galli Immune gene expression in the spleen of chickens experimentally infected with Ascaridia galli](/preview/png/5796753.png)
- Systemic immune gene expression in the spleen of Ascaridia galli infected chickens was assessed by microfluidic qPCR and found to vary at different time points after experimental infection.
- At 2 weeks p.i., lL-13 expression was highly increased in infected chickens as compared to controls.
- At 6 weeks p.i., increased expression of pro-inflammatory cytokine genes was observed in infected chickens.
- A. galli specific IgG was detectable in serum of all infected chickens by 6 weeks p.i.
- A. galli eggs in faeces were detectable in 43% of the infected chickens at week 6 p.i. and in 73% at week 9 p.i.
Ascaridia galli is a gastrointestinal nematode infecting chickens. Chickens kept in alternative rearing systems or at free-range experience increased risk for infection with resulting high prevalences. A. galli infection causes reduced weight gain, decreased egg production and in severe cases increased mortality. More importantly, the parasitised chickens are more susceptible to secondary infections and their ability to develop vaccine-induced protective immunity against other diseases may be compromised. Detailed information about the immune response to the natural infection may be exploited to enable future vaccine development. In the present study, expression of immune genes in the chicken spleen during an experimental infection with A. galli was investigated using the Fluidigm® BioMark⢠microfluidic qPCR platform which combines automatic high-throughput with attractive low sample and reagent consumption. Spleenic transcription of immunological genes was compared between infected chickens and non-infected controls at week 2, 6, and 9 p.i. corresponding to different stages of parasite development/maturation. At week 2 p.i. increased expression of IL-13 was observed in infected chickens. Increased expression of MBL, CRP, IFN-α, IL-1β, IL-8, IL-12β and IL-18 followed at week 6 p.i. and at both week 6 and 9 p.i. expression of DEFβ1 was highly increased in infected chickens. In summary, apart from also earlier reported increased expression of the Th2 signature cytokine IL-13 we observed only few differentially expressed genes at week 2 p.i. which corresponds to the larvae histotrophic phase. In contrast, we observed increased expression of pro-inflammatory cytokines and acute phase proteins in infected chickens, by week 6 p.i. where the larvae re-enter the intestinal lumen. Increased expression of DEFβ1 was observed in infected chickens at week 6 p.i. but also at week 9 p.i. which corresponds to a matured stage where adult worms are present in the intestinal lumen.
Journal: Veterinary Immunology and Immunopathology - Volume 164, Issues 1â2, 15 March 2015, Pages 79-86