کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5797552 | 1555236 | 2016 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Change in vascular smooth muscle response to 5-HT due to short- or long-term endothelial denudation of the bovine digital vein Change in vascular smooth muscle response to 5-HT due to short- or long-term endothelial denudation of the bovine digital vein](/preview/png/5797552.png)
- Endothelial damage has detrimental effects on haemodynamic during laminitis.
- The effect of short/long-term endothelium removal was studied in bovine digital veins.
- Long, but not short, term lack of endothelium induces vascular hyper-reactivity to 5-HT.
- RhoA/ROCK, ROS and COX pathways can account for smooth muscle hyper-reactivity.
Several chronic progressive vascular diseases, such as laminitis, show vasocontractile dysfunction that might evolve into reperfusion injury and/or vessel structural remodelling, which may be traced back to aberrant endothelial function. In the present study, the vasomotor responses of bovine digital veins (BDVs) to 5-hydroxytryptamine (5-HT) were investigated in blood vessels, with and without endothelium present, and in samples deprived of endothelium before or after overnight incubation in tissue culture medium, to evaluate the effects of short- and long-term endothelial damage on vascular smooth muscle (VSM) reactivity. No significant effects were observed in the blood vessels tested immediately after the removal of endothelium. In contrast, a significant increase in VSM reactivity to 5-HT was seen in vessels incubated without endothelium. This long-term change in smooth muscle reactivity was prevented by exposure to the nitric oxide (NO) donor nitroprusside (Pâ<â0.01), suggesting that the long-term lack of inhibitory control exerted by endothelium-derived NO is involved in increased VSM reactivity. The RhoA/ROCK pathway inhibitor fasudil reduced VSM hyper-contractility to ~65% (Pâ<â0.001), the superoxide dismutase-mimetic tempol normalised the vascular response and the non-selective COX-inhibitor indomethacin exerted a moderate inhibitory effect (Pâ<â0.05). Thus, over-activation of the RhoA/ROCK pathway and production of reactive oxygen species could account for VSM hyper-reactivity, triggered by long-term endothelium-deprivation in BDVs, suggesting that these biochemical mechanisms are potential targets for controlling the progressive vasocontractile dysfunction of digital veins in animals affected with laminitis.
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Journal: The Veterinary Journal - Volume 207, January 2016, Pages 154-159