کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5797662 | 1111757 | 2015 | 6 صفحه PDF | دانلود رایگان |
- Two troponin I assays were compared in 23 healthy horses and 72 horses with cardiac disease.
- Large quantitative differences between assays were demonstrated.
- A cut-off value for cardiac disease was identified for only one of the assays.
Cardiac troponin I (cTnI) is a marker for detection of myocardial damage in horses. Many cTnI assays exist and medical studies have shown that the clinical performance of assays differs. The aim of this study was to compare two different cTnI assays in horses. Serum samples were taken from 23 healthy horses (group 1) and 72 horses with cardiac disease (group 2). Cardiac troponin I was determined using assay 1 in laboratory A (limit of detection, LOD, 0.03âng/mL) and assay 2 in laboratories B and C (LOD 0.01âng/mL). In group 1, a median cTnI concentration of <0.03 (<0.03-0.04) ng/mL and <0.01 (<0.01-0.15) ng/mL was found with assays 1 and 2, respectively. A higher median value was demonstrated in group 2 for both assays (assay 1: 0.11âng/mL, range 0.03-58.27âng/mL, Pâ<â0.001; assay 2: 0.02âng/mL, range 0.01-22.87âng/mL, Pâ=â0.044). Although a significant correlation between assays existed, large mean differences that could be important for clinical interpretation of test results were found. A small mean difference was found between laboratories B and C. A significant optimal (Pâ<â0.001) cut-off value for detection of cardiac disease could only be determined for assay 1 (0.035âng/mL, sensitivity 70%, specificity 91%). Assay 1 performed better for detection of cardiac disease in horses in this study.
Journal: The Veterinary Journal - Volume 203, Issue 1, January 2015, Pages 97-102