کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5797689 | 1111758 | 2015 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ex vivo pharmacokinetic and pharmacodynamic analysis of valnemulin against Mycoplasma gallisepticum S6 in Mycoplasma gallisepticum and Escherichia coli co-infected chickens Ex vivo pharmacokinetic and pharmacodynamic analysis of valnemulin against Mycoplasma gallisepticum S6 in Mycoplasma gallisepticum and Escherichia coli co-infected chickens](/preview/png/5797689.png)
- Matrix effects were evaluated in an MIC determination of Mycoplasma gallisepticum.
- Ex vivo PK/PD modeling of valnemulin administered to infected chickens was performed.
- A dosage of 12.4âmg/kg/day valnemulin was calculated for mycoplasmacidal activity.
Pharmacokinetic and pharmacodynamic (PK/PD) indices against Mycoplasma gallisepticum (MG) S6 were investigated in an ex vivo PK/PD model following oral administration of valnemulin to chickens co-infected with M.âgallisepticum and Escherichia coli. The minimum inhibitory concentrations (MICs) for valnemulin against MG S6 in artificial medium and chicken serum were determined. In vitro time-killing curves were established according to a series of multiples of the MIC value in an artificial medium, and ex vivo time-killing curves were established in serum samples obtained from infected chickens at different time points after oral administration with an initial titer of 1âÃâ106 color change units (CCU)/mL MG S6. The sigmoid Emax model was used to provide 24âh area under concentration-time curve/minimum inhibitory concentration ratios (AUC0-24h/MIC) for mycoplasmastasis, mycoplasmacidal activity and mycoplasmal elimination, respectively.The inoculum size and micro or macro methods exhibited little effect on MIC determination of MG, whereas matrix had a large effect. The rapid killing activity observed in in vitro time-killing curves seems to indicate that valnemulin was mycoplasmacidal and concentration dependent against MG. The AUC0-24h/MIC ratio for mycoplasmacidal activity and mycoplasmal elimination was 1321âh and 1960âh, respectively. A dosage regimen of 12.4âmg/kg/day and 18.3âmg/kg/day valnemulin was calculated for mycoplasmacidal activity and mycoplasmal elimination against MG S6, respectively.
Journal: The Veterinary Journal - Volume 204, Issue 1, April 2015, Pages 54-59