کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5894378 1568570 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Flow speed alters the apparent size and concentration of particles measured using NanoSight nanoparticle tracking analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Flow speed alters the apparent size and concentration of particles measured using NanoSight nanoparticle tracking analysis
چکیده انگلیسی
Nanoparticle tracking analysis (NTA) is commonly used to count and size nano-sized particles. A sample loading pump can be used to analyse a larger sample volume, but it is unclear whether accuracy is affected. Using a NanoSight NS300 with the manufacturer-supplied pump, we examined synthetic silica and latex microspheres, liposomes and placental extracellular vesicles at different flow speeds. Analysis at flow speeds of 20 or 50 significantly reduced the measured concentration and mean/modal size of particles, particularly for mono-dispersed samples. We identify sample flow speed as a crucial instrument setting which should be reported in all studies that use NTA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 38, February 2016, Pages 29-32
نویسندگان
, , , , ,