کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5895106 1154447 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Collagen type IV at the fetal-maternal interface
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Collagen type IV at the fetal-maternal interface
چکیده انگلیسی


- Our data suggest that progesterone might regulate collagen type IV.
- Primary extravillous trophoblast cells secrete collagen type IV.
- A novel three-dimensional network of collagen type IV in placenta is presented.
- Trophoblast cells express integrin alpha 10, integrin alpha 11 and collagen receptors DDR-1 and DDR-2.

IntroductionExtracellular matrix proteins play a crucial role in influencing the invasion of trophoblast cells. However the role of collagens and collagen type IV (col-IV) in particular at the implantation site is not clear.MethodsImmunohistochemistry was used to determine the distribution of collagen types I, III, IV and VI in endometrium and decidua during the menstrual cycle and the first trimester of pregnancy. Expression of col-IV alpha chains during the reproductive cycle was determined by qPCR and protein localisation by immunohistochemistry. The structure of col-IV in placenta was examined using transmission electron microscopy. Finally, the expression of col-IV alpha chain NC1 domains and collagen receptors was localised by immunohistochemistry.ResultsCol-IV alpha chains were selectively up-regulated during the menstrual cycle and decidualisation. Primary extravillous trophoblast cells express collagen receptors and secrete col-IV in vitro and in vivo, resulting in the increased levels found in decidua basalis compared to decidua parietalis. A novel expression pattern of col-IV in the mesenchyme of placental villi, as a three-dimensional network, was found. NC1 domains of col-IV alpha chains are known to regulate tumour cell migration and the selective expression of these domains in decidua basalis compared to decidua parietalis was determined.DiscussionCol-IV is expressed as novel forms in the placenta. These findings suggest that col-IV not only represents a structural protein providing tissue integrity but also influences the invasive behaviour of trophoblast cells at the implantation site.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 36, Issue 1, January 2015, Pages 59-68
نویسندگان
, , , , , ,