کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5895338 1154456 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Human trophectoderm apposition is regulated by interferon γ-induced protein 10 (IP-10) during early implantation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Human trophectoderm apposition is regulated by interferon γ-induced protein 10 (IP-10) during early implantation
چکیده انگلیسی

IntroductionThe first step in human implantation is the attraction of the blastocyst to the endometrium. We aimed to study attraction of the human blastocyst to the endometrium, and how this process is accomplished by chemokines secreted by the endometrium.Materials and methodsBlastocyst trophectoderm cells and other trophoblast lineage cells were subjected to attraction assays by IP-10 and other chemokines using transwell migration and chemotaxis assays. Chemokine expression and secretion were investigated using immunohistochemistry, ELISA, FACS analysis, and RT-PCR on material from flushing of the uterine cavity in endometrial biopsies. Chemokine receptor expression by blastocyst trophectoderm following PGD biopsy, trophectoderm derived from hES, placental villi, and other trophoblast lineage cells were characterized by the same methods.ResultsIP-10 dramatically attracted trophectoderm derived from hES cells and other lineages by interaction with CXCR3 chemokine receptors, as shown by both chemotaxis and transwell migration. High levels of IP-10 were detected throughout the menstrual cycle at flushing of the uterine cavity. Immunohistochemistry, FACS analysis, and RT-PCR of endometrial biopsy detected IP-10 in glandular and stromal cells of the endometrium. High levels of IP-10 were detected in condition medium of the endometrial stromal and glandular cells. Of all of the chemokine/chemokine receptor combinations examined, the IP-10/CXCR3 interaction was the only cytokine that was significantly elevated.DiscussionWhile they await the wandering blastocyst, IP-10 is produced by many cells of the endometrium, but not by endometrial natural killer cells.ConclusionEndometrial IP-10 may specifically attract human blastocyst trophectoderm cells early in implantation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 34, Issue 3, March 2013, Pages 222-230
نویسندگان
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