کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905175 | 1159856 | 2016 | 8 صفحه PDF | دانلود رایگان |
- We screened a sponge-associated bacterial metagenome library for novel cadmium accumulation genes.
- The discovered Cd accumulation protein, Cdae-1, although coupled with a signal peptide, promoted intracellular Cd accumulation.
- Cdae-1 showed different amino acid features and characteristics to metallothioneins and phytochelatins.
- Cdae-1 harbored a novel pentapeptide motif unique to a class of hypothetical or low-complexity proteins of unknown function.
Metagenome research has brought much attention for the identification of important and novel genes of industrial and pharmaceutical value. Here, using a metagenome library constructed from bacteria associated with the marine sponge, Styllisa massa, a high-throughput screening technique using radioisotope was implemented to screen for cadmium (Cd) binding or accumulation genes. From a total of 3301 randomly selected clones, a clone 247-11C was identified as harboring an open reading frame (ORF) showing Cd accumulation characteristics. The ORF, termed as ORF5, was further analyzed by protein functional studies to reveal the presence of a protein, Cdae-1. Cdae-1, composed of a signal peptide and domain harboring an E(G/A)KCG pentapeptide motif, enhanced Cd accumulation when expressed in Escherichia coli. Although showing no direct binding to Cd in vitro, the presence of important amino acid residues related to Cd detoxification suggests that Cdae-1 may possess a different mechanism from known Cd binding proteins such as metallothioneins (MTs) and phytochelatins (PCs). In summary, using the advantage of bacterial metagenomes, our findings in this work suggest the first report on the identification of a unique protein involved in Cd accumulation from bacteria associated with a marine sponge.
Journal: Gene - Volume 576, Issue 2, Part 1, 1 February 2016, Pages 618-625