کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5905432 1159891 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single nucleotide polymorphism creating a variable upstream open reading frame regulates glucocorticoid receptor expression
ترجمه فارسی عنوان
پلیمورفیسم تک نوکلئوتید ایجاد یک چارچوب خواندن مجدد متغیر بالادست بالا، بیانگر گیرنده گلوکوکورتیکوئید است
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی
The glucocorticoid and mineralocorticoid receptors are known to play a crucial role in cellular responses to acute and chronic stress conditions. However, the influence of genetic variants and regulatory mechanisms within the glucocorticoid and mineralocorticoid receptor genes NR3C1 and NR3C2 is still incompletely understood. We therefore investigated putative upstream open reading frames, a motif regulating gene expression, from the 5′ untranslated regions of the predominant human glucocorticoid receptor gene NR3C1 isoform alpha variant 1 and from the human mineralocorticoid receptor NR3C2 variants 1 and 2. The in silico analysis displayed one SNP (rs10482612), being present heterozygously in about 1.2% of the world population and 1.8% of the European population (according to the NCBI database), whose minor allele 'A' creates an upstream start codon. Our functional analysis performed by reporter gene assay and quantitative real-time PCR confirmed that the minor allele 'A' of the SNP rs10482612 can indeed alter protein activity of the subsequent gene during baseline conditions and cellular stress by creating a functional uORF in the 5′UTR of the NR3C1 transcript variant 1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 563, Issue 1, 25 May 2015, Pages 24-28
نویسندگان
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