کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905458 | 1159895 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Haplotype combination of the bovine CFL2 gene sequence variants and association with growth traits in Qinchuan cattle
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کلمات کلیدی
PICQinchuan cattleHaplotype combinationSequence variants - انواع توالیPCR-RFLP - روش PCR-RFLPGrowth traits - صفات رشدLinkage disequilibrium - عدم تعادل پیوستگیpolymorphism information content - محتوای اطلاعات پلی مورفیسمUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهrestriction fragment length polymorphism - پلی مورفیسم طول قطعه قطعهSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this study was to examine the association of cofilin2 (CFL2) gene polymorphisms with growth traits in Chinese Qinchuan cattle. Three single nucleotide polymorphisms (SNPs) were identified in the bovine CFL2 gene using DNA sequencing and (forced) PCR-RFLP methods. These polymorphisms included a missense mutation (NC_007319.5: g. C 2213 G) in exon 4, one synonymous mutation (NC_007319.5: g. T 1694 A) in exon 4, and a mutation (NC_007319.5: g. G 1500 A) in intron 2, respectively. In addition, we evaluated the haplotype frequency and linkage disequilibrium coefficient of three sequence variants in 488 individuals in QC cattle. All the three SNPs in QC cattle belonged to an intermediate level of genetic diversity (0.25Â <Â PICÂ <Â 0.5). Haplotype analysis of three SNPs showed that 8 different haplotypes were identified in all, but only 5 haplotypes were listed except for those with a frequency of <Â 0.03. Hap4 (-GTC-) had the highest haplotype frequencies (34.70%). However in the three SNPs there were no significant associations between the 13 combined genotypes of the CFL2 gene and growth traits. LD analysis showed that the SNP T 1694 A and C 2213 G loci had a strong linkage (r2Â >Â 0.33). Association analysis indicated that SNP G 1500 A, T 1694 A and C 2213 G were significantly associated with growth traits in the QC population. The results of our study suggest that the CFL2 gene may be a strong candidate gene that affects growth traits in the QC cattle breeding program.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 563, Issue 2, 1 June 2015, Pages 136-141
Journal: Gene - Volume 563, Issue 2, 1 June 2015, Pages 136-141
نویسندگان
Yujia Sun, Xianyong Lan, Chuzhao Lei, Chunlei Zhang, Hong Chen,