کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905466 | 1159895 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning, expression analysis, and appetite regulatory effect of peptide YY in Siberian sturgeon (Acipenser baerii)
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کلمات کلیدی
DPP-IVPYYAcipenser baeriiintraperitoneal injection - تزریق داخل صفاقیrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدdipeptidyl peptidase IV - دیپپتیدیل پپتیداز IVCITES - سیتسFood deprivation - محرومیت از مواد غذاییRace - مسابقهfood intake - مصرف غذاbody weight - وزن بدنpeptide YY - پپتید YYhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراConvention on International Trade in Endangered Species - کنوانسیون تجارت بین المللی گونه های در معرض خطر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular cloning, expression analysis, and appetite regulatory effect of peptide YY in Siberian sturgeon (Acipenser baerii) Molecular cloning, expression analysis, and appetite regulatory effect of peptide YY in Siberian sturgeon (Acipenser baerii)](/preview/png/5905466.png)
چکیده انگلیسی
Peptide YY (PYY) is an anorectic brain-gut peptide involved in feeding regulation and well characterized in mammals. However, the functional role of PYY in the appetite regulatory of fish is not clear. In this study, we characterized a high conservation of PYY cDNA and found high expression levels of PYY mRNA in the brain and digestive tract of Siberian sturgeon. Then, we examined preprandial (pre- and post-feeding) changes of PYY mRNA expression in the brain that showed a significantly increased in 3Â h post-feeding, suggesting an anorectic possible function of PYY in Siberian sturgeon. Next, we examined the expression of PYY mRNA during 15Â days fasting and refeed after fasting. The SsPYY mRNA expression of unfed fish had a significant 2.4, 1.7, 2.0, 2.2, and 2.1-fold decrease compared to 1-, 3-, 6-, 10- and 15-day ad libitum fed animals, respectively. After refeed, SsPYY mRNA significantly increased 1.9 and 4.1-fold above that of the 15-day fed and unfed fish control group (PÂ <Â 0.01). Furthermore, a single intraperitoneal injection of 10, 100 and 200Â ng/g BW SsPYY1-36 caused a reduction in the next feeding and no significant reduction in food intake was observed in fish injected with a 1Â ng/g BW. Overall, PYY has a potentially role in food intake attenuation of Siberian sturgeon.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 563, Issue 2, 1 June 2015, Pages 172-179
Journal: Gene - Volume 563, Issue 2, 1 June 2015, Pages 172-179
نویسندگان
Hu Chen, Xin Zhang, Jin Hao, Defang Chen, Ju Liu, Yundi Gao, Jieyao Zhu, Hongwei Wu, Fangjun Lin, Yundan Pu, Dengyue Yuan, Rongbin Wei, Chaowei Zhou, Tao Wang, Zhiqiong Li,