کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905508 | 1159902 | 2014 | 7 صفحه PDF | دانلود رایگان |

- AmphiCFH gene was cloned from Branchiostoma belcheri with a 1295Â bp cDNA product.
- AmphiCFH protein has similar conserved domains with human CFH.
- AmphiCFH gene was ubiquitously and differentially expressed in investigated tissues.
- AmphiCFH gene was involved in the innate immune response of LPS stimulation.
- AmphiCFH gene was the ancestor of the vertebrate CFH gene.
Complement factor H (CFH) is an essential regulator of the complement system and plays very important roles in animal innate immunity. Although the complement system of amphioxus has been extensively studied, the expression in amphioxus and evolution of CFH gene remain unknown. In this study, we identified and characterized an amphioxus (Branchiostoma belcheri) CFH gene (designated as AmphiCFH). Our results showed that the full-length cDNA of AmphiCFH gene consists of 1295Â bp nucleotides containing an 855Â bp open reading frame (ORF) that was predicted to encode a 284 amino acid protein. The putative AmphiCFH protein possessed the characteristic of the CFH protein family, including typical CCP (complement control protein) domain. Real-time PCR analysis showed that the AmphiCFH was ubiquitously and differentially expressed in five investigated tissues (intestine, gills, notochord, muscles, and hepatic cecum). The expression level of the AmphiCFH gene was induced upon lipopolysaccharide stimulation, indicating that the AmphiCFH gene might be involved in innate immunity. In addition, phylogenetic analysis showed that the AmphiCFH gene was located between that of invertebrates and vertebrates, suggesting that the AmphiCFH gene is a member of the CFH gene family. In conclusion, our findings provided an insight into animal innate immunity and evolution of the CFH gene family.
Journal: Gene - Volume 553, Issue 1, 10 December 2014, Pages 42-48