کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905572 | 1159908 | 2015 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and expression characterization of the myostatin (MSTN) gene and association analysis with growth traits in the razor clam Sinonovacula constricta
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کلمات کلیدی
MSTNORFqRT-PCRTGFSinonovacula constricta - انقباض Sinonovaculatransforming growth factor - تبدیل فاکتور رشدrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدGrowth - رشدShell length - طول شلopen reading frame - قاب خواندن بازRace - مسابقهUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهMyostatin - میوستاتینquantitative reverse transcription polymerase chain reaction - واکنش زنجیره ای پلی مراز رونویسی معکوسbody weight - وزن بدنSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification and expression characterization of the myostatin (MSTN) gene and association analysis with growth traits in the razor clam Sinonovacula constricta Identification and expression characterization of the myostatin (MSTN) gene and association analysis with growth traits in the razor clam Sinonovacula constricta](/preview/png/5905572.png)
چکیده انگلیسی
Myostatin (MSTN) is a member of the transforming growth factor-β superfamily (TGF-β) and is an important negative regulator of muscle growth in vertebrates. In this study, we cloned and analyzed the MSTN gene (Sc-MSTN) from razor clam (Sinonovacula constricta). The full length of Sc-MSTN cDNA sequence consists of 4226 base pairs (bp), comprising a 522-bp 5â² untranslated region (UTR), a 2342-bp 3â²UTR, and an open reading frame (ORF) that is 1362 in length. The ORF encodes 453 amino acids with a RXXR proteolytic site and nine conserved cysteines. Quantitative real-time PCR analysis revealed that the Sc-MSTN transcript was expressed in a wide range of tissues but appeared to exhibit the greatest level of expression in the foot. The transcript was widely detected in early developmental stages, showing the highest expression in the trochophore stage. Furthermore, six SNPs were identified in the coding region of the Sc-MSTN gene using direct sequencing. SNP-1 is non-synonymous and involves an amino acid change from Leu to Ser. Association analysis showed that SNP-1 and SNP-6 had significant influences on shell length (SL). The results suggested that MSTN could be selected as a candidate gene for the future molecular breeding of razor clam strains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 297-304
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 297-304
نویسندگان
Donghong Niu, Lie Wang, Zhiyi Bai, Shumei Xie, Honggang Zhao, Jiale Li,