کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905597 | 1159908 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dinitrophenol modulates gene expression levels of angiogenic, cell survival and cardiomyogenic factors in bone marrow derived mesenchymal stem cells
ترجمه فارسی عنوان
دینیتروفنول مدولا بیان سطح ژن آنژیوژنیک، بقای سلولی و عوامل قلب و عروق در سلول های بنیادی مزانشیمی مغز استخوان
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کلمات کلیدی
NRP1ankyrin repeat domain 1EREGANKRD1MyogeninEpiregulinJAG1KDRJagged 1EndoglinPGFENGDNPCTGFMSCsTEK - تکRe-oxygenation - دوباره اکسیژن کردنMesenchymal stem cells - سلول های بنیادی مزانشیمیCytokines - سیتوکین هاplacental growth factor - عامل رشد جفتGrowth factors - فاکتور رشدConnective tissue growth factor - فاکتور رشد بافت همبندMYOG - منوNeuropilin - نوروپیلینPreconditioning - پیش شرط بندیkinase insert domain receptor - کیناز گیرنده دامنه را وارد کنید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
چکیده انگلیسی
Various preconditioning strategies influence regeneration properties of stem cells. Preconditioned stem cells generally show better cell survival, increased differentiation, enhanced paracrine effects, and improved homing to the injury site by regulating the expression of tissue-protective cytokines and growth factors. In this study, we analyzed gene expression pattern of growth factors through RT-PCR after treatment of mesenchymal stem cells (MSCs) with a metabolic inhibitor, 2,4 dinitrophenol (DNP) and subsequent re-oxygenation for periods of 2, 6, 12 and 24Â h. These growth factors play important roles in cardiomyogenesis, angiogenesis and cell survival. Mixed pattern of gene expression was observed depending on the period of re-oxygenation. Of the 13 genes analyzed, ankyrin repeat domain 1 (Ankrd1) and GATA6 were downregulated after DNP treatment and subsequent re-oxygenations. Ankrd1 expression was, however, increased after 24Â h of re-oxygenation. Placental growth factor (Pgf), endoglin (Eng), neuropilin (Nrp1) and jagged 1 (Jag1) were up-regulated after DNP treatment. Gradual increase was observed as re-oxygenation advances and by the end of the re-oxygenation period the expression started to decrease and ultimately regained normal values. Epiregulin (Ereg) was not expressed in normal MSCs but its expression increased gradually from 2 to 24Â h after re-oxygenation. No change was observed in the expression level of connective tissue growth factor (Ctgf) at any time period after re-oxygenation. Kindlin3, kinase insert domain receptor (Kdr), myogenin (Myog), Tbx20 and endothelial tyrosine kinase (Tek) were not expressed either in normal cells or cells treated with DNP. It can be concluded from the present study that MSCs adjust their gene expression levels under the influence of DNP induced metabolic stress. Their levels of expression vary with varying re-oxygenation periods. Preconditioning of MSCs with DNP can be used for enhancing the potential of these cells for better regeneration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 448-457
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 448-457
نویسندگان
Anwar Ali, Muhammad Aleem Akhter, Kanwal Haneef, Irfan Khan, Nadia Naeem, Rakhshinda Habib, Nurul Kabir, Asmat Salim,