کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905608 | 1159909 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of E3 ubiquitin ligase neuregulin receptor degradation protein-1 (Nrdp1) in the large yellow croaker (Larimichthys crocea) and its immune responses to Cryptocaryon irritans
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کلمات کلیدی
ORFdeoxyribonucleaseEAELarimichthys croceaNrdp1cDNA - cDNADNA complementary to RNA - DNA مکمل RNADNAse - DNAaseexperimental allergic encephalomyelitis - آنسفالومیلیت آلرژیک تجربیopen reading frame - قاب خواندن بازUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهImmune response - پاسخ یا واکنش ایمنیCryptocaryon irritans - کریپتوکاریون اریتتین ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Neuregulin receptor degradation protein-1 (Nrdp1) was recently identified in humans as an important immune factor responding to the challenge of virus, LPS or cytokine. Its role in fish immune defense and whether it is involved in anti-parasite immunity have not been proven yet. In this report, the full-length cDNA sequence and genomic structure of Nrdp1 in the large yellow croaker Larimichthys crocea (LcNrdp1) were identified and characterized. The full-length cDNA of LcNrdp1 was 1248Â bp, including a 5â² untranslated region (UTR) of 32Â bp, a 3â² UTR of 259Â bp and an open reading frame (ORF) of 937Â bp, encoding a polypeptide of 318 amino acid residues. The full-length genomic DNA sequence of LcNrdp1 was composed of 2635 nucleotides, including four exons and three introns. The putative LcNrdp1 protein had no signal peptide sequence and contained a characteristic Nrdp1 consensus motif C3HC3D ring finger and a Coiled-coil domain. Phylogenetic analysis showed that Nrdp1 in fish was closer with that in other vertebrates (79%-90% amino acid identity) than in invertebrates and bacteria (27%-65%). In fishes, Nrdp1 in large yellow croaker was closer with that in Takifugu rubripes. The expression profile showed that LcNrdp1 was constitutively expressed in all tested tissues, especially highly expressed in brain, muscle and kidney. Post-infection (PI) with Cryptocaryon irritans, an increased expression of LcNrdp1 was induced in infection sites (skin and gill), whereas in immune organs, the expression of LcNrdp1 was up-regulated in spleen (except the 1st d and 10th d PI) but suppressed in head kidney. These results suggested that LcNrdp1 might play an important immune role in the finfish L. crocea in the defense against the parasite C. irritans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 556, Issue 2, 10 February 2015, Pages 98-105
Journal: Gene - Volume 556, Issue 2, 10 February 2015, Pages 98-105
نویسندگان
Dong Ling Zhang, Fang Han, Da Hui Yu, Shi Jun Xiao, Ming Yun Li, Jian Chen, Zhi Yong Wang,