کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5906425 1159970 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Major cis-regulatory elements for rice bidirectional promoter activity reside in the 5′-untranslated regions
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Major cis-regulatory elements for rice bidirectional promoter activity reside in the 5′-untranslated regions
چکیده انگلیسی


- Bidirectional promoter activity was dissected in three rice promoters.
- 5'-untranslated regions of divergent genes harbor bidirectional activity.
- A novel mechanism is proposed for activity of bidirectional promoters.

Bidirectional promoters are defined as those that regulate adjacent genes organized in a divergent fashion (head to head orientation) and separated by < 1 kb. In order to dissect bidirectional promoter activity in a model plant, deletion analysis was performed for seven rice promoters using promoter-reporter gene constructs, which identified three promoters to be bidirectional. Regulatory elements located in or close to the 5′-untranslated regions (UTR) of one of the genes (divergent gene pair) were found to be responsible for their bidirectional activity. DNA footprinting analysis identified unique protein binding sites in these promoters. Deletion/alteration of these motifs resulted in significant loss of expression of the reporter genes on either side of the promoter. Changes in the motifs at both the positions resulted in a remarkable decrease in bidirectional activity of the reporter genes flanking the promoter. Based on our results, we propose a novel mechanism for the bidirectionality of rice bidirectional promoters.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 526, Issue 2, 10 September 2013, Pages 400-410
نویسندگان
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