Comparative genomic analysis and evolution of family-B G protein-coupled receptors from six model insect species

► Genome-wide survey revealed 87 family-B GPCRs from six model insects. ► Insect GPCR-Bs exhibited three kinds of evolutionary patterns. ► pthrl was discovered in invertebrates firstly. ► Osmoregulated HE6 receptor arose species-specific duplication in T. castaneum. ► mthl largely expanded in D. melanogaster likely due to adaptive evolution.