Investigating the effects of the presence of foreign DNA on DNA methylation and DNA repair events in cultured eukaryotic cells

► Vector insertion in 143B cell line decreases DNA methylation in MGMT-gene promoter ► Altered DNA methylation of the p16INK4a-gene promoter observed ► Deviations in the stable gene expression due to a decrease in transgene mRNA ► Observed variation in promoter methylation may imply unstable protein expression. ► Foreign DNA insertion into genome of cultured cells manifest in their metabolism.