کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5907122 1160000 2012 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Campylobacter fetus subspecies: Comparative genomics and prediction of potential virulence targets
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Campylobacter fetus subspecies: Comparative genomics and prediction of potential virulence targets
چکیده انگلیسی

The genus Campylobacter contains pathogens causing a wide range of diseases, targeting both humans and animals. Among them, the Campylobacter fetus subspecies fetus and venerealis deserve special attention, as they are the etiological agents of human bacterial gastroenteritis and bovine genital campylobacteriosis, respectively. We compare the whole genomes of both subspecies to get insights into genomic architecture, phylogenetic relationships, genome conservation and core virulence factors. Pan-genomic approach was applied to identify the core- and pan-genome for both C. fetus subspecies and members of the genus. The C. fetus subspecies conserved (76%) proteome were then analyzed for their subcellular localization and protein functions in biological processes. Furthermore, with pathogenomic strategies, unique candidate regions in the genomes and several potential core-virulence factors were identified. The potential candidate factors identified for attenuation and/or subunit vaccine development against C. fetus subspecies contain: nucleoside diphosphate kinase (Ndk), type IV secretion systems (T4SS), outer membrane proteins (OMP), substrate binding proteins CjaA and CjaC, surface array proteins, sap gene, and cytolethal distending toxin (CDT). Significantly, many of those genes were found in genomic regions with signals of horizontal gene transfer and, therefore, predicted as putative pathogenicity islands. We found CRISPR loci and dam genes in an island specific for C. fetus subsp. fetus, and T4SS and sap genes in an island specific for C. fetus subsp. venerealis. The genomic variations and potential core and unique virulence factors characterized in this study would lead to better insight into the species virulence and to more efficient use of the candidates for antibiotic, drug and vaccine development.

► Campylobacter fetus subspecies have 76% genome similarity. ► The C. fetus subspecies core genome contains 1628 gene families. ► Pathogenicity islands predicted in C. fetus subspecies. ► Virulence factors and vaccine candidates in C. fetus subspecies identified. ► Potential Immunogenic core-exoproteins have been identified.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 508, Issue 2, 25 October 2012, Pages 145-156
نویسندگان
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