Delivery of AP-2α siRNA into cultured bovine trophoblast cells by electroporation repressed key placenta-specific gene expression

► We established a cell culture model for bovine trophoblast cells. ► siRNA duplexes against TFAP2A was effectively transfected into the cells. ► Electroporating siRNA into bovine trophoblast cells could be an efficient method. ► AP-2α could be involved in regulation of expression CSH1 and PRP1 transcripts.