Mechanism of entomotoxicity of the plant lectin from Hippeastrum hybrid (Amaryllis) in Spodoptera littoralis larvae

► The plant lectin HHA affects Spodoptera littoralis larval growth and development. ► HHA binds to S. littoralis midgut columnar cells through mannose-containing glycan structures. ► HHA endocytosis in S. littoralis midgut columnar cells is partially clathrin-mediated. ► HHA interaction with midgut cells interferes with nutrient absorption. ► This study confirms the potential of mannose-specific lectins as pest control agents.