Fluorescence competition assay for the assessment of green leaf volatiles and trans-β-farnesene bound to three odorant-binding proteins in the wheat aphid Sitobion avenae (Fabricius)

► Fluorescent competition assay help reveal OBPs' functions in Sitobion avenae antennae. ► We cloned three specific SaveOBPs with complete coding regions. ► The binding data of the three OBPs to dozens of wheat volatiles have been shown. ► SaveOBP2 and SaveOBP3 exhibited a 'general' binding property to green leaf volatiles. ► SaveOBP7 showed a strong binding affinity to trans-β-farnesene.