From individual to collective immunity: The role of the venom as antimicrobial agent in the Stenogastrinae wasp societies

Sociality is associated with an increased risk of disease transmission and one of the first defense of the insect colonies is represented by antimicrobial secretions. In many eusocial hymenopteran species venom glands represent one of the most important source of antimicrobial substances. It is known that in highly eusocial species the venom is spread on both the cuticle of insects and the comb, thus becoming a component of the so called “social immunity”. So far, it is never been ascertained whether this phenomenon is also present in more primitively eusocial and incipiently eusocial groups. Using incipiently eusocial hover wasps as model, we demonstrate that venom is present on insect cuticles and that it strongly acts against microorganisms. By contrast, the nest, regardless of materials, does not represent a ''medium” where the venom is deposited by wasps in order to act as a social antiseptic weapon. Our findings discussed in an evolutionary perspective indicate that a certain degree of sociality or a sufficient number of individuals in an insect society are thresholds to be reached for the rise of complex and efficient forms of collective and social immunity as mechanisms of resistance to diseases.

Highlights► We demonstrated the strong antimicrobial activity of the venom of Stenogastrinae wasps. ► We found that the antimicrobial venom is present on the cuticle of the wasps. ► By contrast the venom is not deposited by wasps on the nests. ► Nest is not used as social antiseptic device as in other highly eusocial insects. ► “Social immunity” mechanisms require a certain degree of sociality to rise.