Elongation of in vitro cultured ovarian follicles in the mosquito, Culex pipiens pallens

► Stage III mosquito ovarian follicles are cultured in PBS-based medium for 24-30 h. ► Time-lapse movies showed dynamic follicle elongation from oval to long mature shape. ► Cytochalasin D or colchicine inhibited normal follicle elongation. ► Actin fibers or microtubules might be involved in this active morphological change.