کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5926111 | 1167338 | 2013 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nitric oxide plays a minimal role in hypoxic pulmonary vasoconstriction in isolated rat lungs
ترجمه فارسی عنوان
اکسید نیتریک نقش کمتری را در ریزش موی هیپوکسیک ریه در ریه های جدا شده موش نقش می کند
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
فیزیولوژی
چکیده انگلیسی
The goal of this study was to elucidate the importance of nitric oxide production during hypoxic pulmonary vasoconstriction (HPV). One group of Sprague Dawley rats received an ip injection of saline (controls), while a second group received an ip injection of Escherichia coli lipopolysacharides (LPS-treated) to render them septic. Three hours later, the animals were anesthetized and prepared for the isolated lung experiment. The lungs were ventilated and perfused with diluted autologous blood (Hct 23%) at constant flow rate while monitoring pulmonary arterial pressure (Pa). Nitric oxide production from the lungs was monitored by measuring its concentration in the mixed exhaled gas (NOe) offline. NOe in the isolated lungs was 2Â ppb in controls and 90Â ppb in the LPS treated lungs. Hypoxia caused Pa to rise from 10 to 17Â mmHg in control lungs, and from 10 to 27Â mmHg in the LPS treated lungs. NO production was then manipulated to determine if it affects HPV. NOe was increased by adding l-arginine to the blood, and was blocked by adding nitro-l-arginine (LNA). l-Arginine had minimal effect on NOe in control lungs, but increased NOe in LPS treated lungs, and yet HPV was similar in the 2 groups. Despite inhibition of NO synthesis with nitro-l-arginine (LNA), HPV was potentiated equally in control and in LPS treated lungs (Pa rose by 23Â mmHg). Thus NO production did not affect the difference in HPV between control and LPS treated lungs. The results suggest that NO does not plays a primary role in HPV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Respiratory Physiology & Neurobiology - Volume 189, Issue 1, 1 October 2013, Pages 93-98
Journal: Respiratory Physiology & Neurobiology - Volume 189, Issue 1, 1 October 2013, Pages 93-98
نویسندگان
T.S. Hakim, A. Pedoto, D. Mangar, E.M. Camporesi,