Gold nanoparticles-decorated silver-bipyridine nanobelts for the construction of mediatorless hydrogen peroxide biosensor

• Novel gold nanoparticles decorated silver:bipyridine nanobelts are reported.
• Decorated nanobelts showed high electrocatalytic activity toward H2O2.
• A sensitive nanostructured enzyme biosensors for H2O2 is reported.

Au nanoparticles modified with 4-mercaptopyridine and 6-mercapto-1-hexanol were used as coordination agents to prepare a novel hybrid nanomaterial with Ag:4,4′-bipyridine nanobelts. This nanohybrid was employed to modify glassy carbon electrodes and to construct a horseradish peroxidase-based mediatorless amperometric biosensor for H2O2. The electrode, poised at −100 mV, exhibited a rapid response within 4 s and a linear calibration range from 90 pM to 6.5 nM H2O2. The biosensor showed a high sensitivity of 283 A/M cm2 and a very low detection limit of 45 pM at a signal-to-noise ratio of 3. The enzyme biosensor showed high stability when stored at 4 °C under dry conditions, retaining over 96% and 78% of its initial activity after 15 and 30 days of storage at 4 °C, respectively.

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