A simple one-step method for preparation of fluorescent carbon nanospheres and the potential application in cell organelles imaging

• A simple one step method was developed to prepare CNP from biomaterials.
• Cell uptake of CNP is a concentration-dependent manner.
• CNP showed poor colocalization with endosomes and mitochondria.
• CNP could adsorb serum protein.
• CNP showed well hemocompatibility and low cytotoxicity.

Highly fluorescent carbon nanospheres with a quantum yield of 17.6% have been prepared by a one-step method with hydrothermal treatment of spider silk. Due to the high photostability, low toxicity and well blood compatibility, these carbon nanospheres could be used as an excellent probes for cancer cell imaging.

Figure optionsDownload high-quality image (61 K)Download as PowerPoint slide