کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
607369 1454576 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hydroxyl density affects the interaction of fibrinogen with silica nanoparticles at physiological concentration
ترجمه فارسی عنوان
تراکم هیدروکسیل اثر متقابل فیبرینوژن با نانوذرات سیلیکا را در غلظت فیزیولوژیک تأثیر می گذارد
کلمات کلیدی
هیدرولیکی سطح تراکم سیلانول، سیلیس آمورف، تعاملات فیبرینوژن
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی


• Silanols density affects the interaction of fibrinogen with silica nanoparticles.
• At physiological concentration fibrinogen self-assembles at the silica surface.
• The hydrophilic degree tunes the fibrinogen self-assembly.
• Fibrinogen induce silica nanoparticles agglomeration.

An increasing interest in the interaction between blood serum proteins and nanoparticles has emerged over the last years. In fact, this process plays a key role in the biological response to nanoparticles. The behavior of proteins at the biofluid/material interface is driven by the physico-chemical properties of the surface. However, much research is still needed to gain insight into the process at a molecular level.In this study, the effect of silanol density on the interaction of fibrinogen at physiological concentrations with silica nanoparticle/flat surfaces has been studied.Silica nanoparticles and silica wafers were modified and characterized to obtain a set of samples with different silanols density. The interaction with fibrinogen has been studied by evaluating the extent of coverage (bicinchoninic acid assay) and the irreversibility of adsorption (shift of the ζ potential). To clarify the molecular mechanism of fibrinogen/surface interactions, confocal micro-Raman spectroscopy (nanoparticles) and atomic force microscopy (wafers) were used. Finally the effect of fibrinogen on the agglomeration of nanoparticles has been evaluated by Flow Particle Image Analysis.The data reported here show that a minimal variation in the state of the silica surface modifies the adsorption behavior of fibrinogen, which appears mediated by a competition between protein/protein and protein/surface interactions. By comparing the data obtained on nanoparticles and silicon-supported silica layers, we found that hydrophilicity increases the tendency of fibrinogen molecules to interact with the surface rather than with other molecules, thus inhibiting fibrinogen self-assembly.This study contributes to the knowledge of the processes occurring at the surface/biological fluids interface, needed for the design of new biocompatible materials.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 419, 1 April 2014, Pages 86–94
نویسندگان
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