Cellular and molecular investigations of the adhesion and mechanics of Listeria monocytogenes lineages’ I and II environmental and epidemic strains

Atomic force microscopy (AFM) was used to probe the mechanical and adherence properties of eight Listeriamonocytogenes’ strains representative of the species’ two phylogenetic lineages I and II. From a functional perspective, lineage’ I strains were characterized by lower overall adhesion forces and higher specific and nonspecific forces compared to lineage’ II strains. From a structural perspective, lineage’ II strains were characterized by higher Young’s moduli and longer and stiffer biopolymers compared to lineage’ I strains. Both lineages’ I and II strains were similar in their grafting densities. Finally, our results indicated that epidemic and environmental strains of L. monocytogenes and irrespective of their lineage group were characterized by similar Young’s moduli of elasticities and adhesion forces at the cellular level. However, at the molecular level, epidemic strains were characterized by higher specific and nonspecific forces, shorter, denser, and more flexible biopolymers compared to environmental strains.

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► Lineage’ I strains were characterized by shorter and more flexible biopolymers.
► Lineage’ II strains were characterized by higher adhesion strengths to Si3N4.
► Lineage’ I strains were characterized by higher specific and nonspecific forces.
► Bacterial elasticities were similar for epidemic and environmental strains.
► Epidemic strains were characterized by short, dense and collapsed biopolymers.