کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
608333 880576 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Promoting immobilization and catalytic activity of horseradish peroxidase on mesoporous silica through template micelles
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Promoting immobilization and catalytic activity of horseradish peroxidase on mesoporous silica through template micelles
چکیده انگلیسی

New concept on the promotion of immobilization and catalytic activity of enzyme on mesoporous silica through template micelles is proposed and realized in this paper. Proper P123 templates are controllable retained in the as-synthesized SBA-15, not only to anchor the horseradish peroxidase (HRP) guest, but also to establish the crowding-like microenvironment around the enzyme. The influence of retaining templates on the pore structure of SBA-15, immobilization, and catalytic activity of HRP is studied, and the possible process of template removal is proposed. Ethanol refluxing of 6 h is conformable to prepare the optimal mesoporous support characterized with the retained templates of about 8%. With the assistance of retained templates in SBA-15, up to 49 mg g−1 of HRP can be immobilized, 100% more than that on calcined SBA-15. Furthermore, the thermal stability, the resistance of pH variation and denaturing agent urea, and the recycle usage of HRP immobilized are obviously elevated, paving a novel and low-cost route to develop enzyme catalysts.

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► The templates of SBA-15 are exploited to make the optimal environment for enzyme.
► More HRP can be immobilized, up to 49 mg g−1, in the SBA-15 reserved P123 templates.
► Thermal stability of HRP/SBA-15 is greatly enhanced.
► The HRP immobilized kept 74% of original activity after used for 5 times.
► This new strategy converts the scrap micelles to efficient modifier for enzyme catalysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 377, Issue 1, 1 July 2012, Pages 497–503
نویسندگان
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