Synthesis of graphene oxide-based biocomposites through diimide-activated amidation

In this work, a novel and facile method for covalent attachment of biomaterials to graphene oxide sheets (GOS) was developed. Four conjugates were obtained via the diimide-activated amidation reaction under ambient conditions. Final products were characterized by FT-IR spectroscopy, atomic force microscopy and transmission electron microscopy. Electrochemical characterization of the composite showed that the covalently bonded biomaterial retained its bioactivity. This method may provide a way for further preparation of graphene-based biodevices.

A novel and facile method for attachment of biomaterials to graphene oxide sheets was developed. The covalently bonded biomaterial retained its bioactivity.Figure optionsDownload high-quality image (110 K)Download as PowerPoint slideResearch highlights
► Diimide-activated amidation was used to attach biomaterials to graphene oxide sheets.
► It causes no denaturing of the biomaterials and guaranties the uniform attachment.
► The same method can be used to introduce GOS into other biosystems.