کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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609858 | 880633 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Amperometric immunosensor for the determination of α-1-fetoprotein based on multiwalled carbon nanotube–silver nanoparticle composite Amperometric immunosensor for the determination of α-1-fetoprotein based on multiwalled carbon nanotube–silver nanoparticle composite](/preview/png/609858.png)
A new amperometric immunosensor for the determination of α-1-fetoprotein (AFP) has been constructed. First, a multiwalled carbon nanotube–silver (MWNT–Ag) composite was modified on the surface of a glassy carbon electrode. Then, chitosan–MnO2 (CS–MnO2) with excellent film forming ability was dipped onto the MWNT–Ag-modified electrode. Subsequently, gold nanoparticles were electrodeposited on the electrode to immobilize anti-AFP. The assembly processes were characterized with cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. MWNT–Ag composite was characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, and UV–vis spectroscopy. The system was optimized to realize a reliable determination of AFP in the range of 0.25–250 ng/ml with a detection limit of 0.08 ng/ml (S/N = 3). The proposed immunosensor showed a rapid and highly sensitive amperometric response to AFP with acceptable stability and reproducibility.
The schematic illustration of stepwise fabrication process of the immunosensor: BSA/anti-AFP/CS–MnO2/MWNTs–Ag/GCE.Figure optionsDownload high-quality image (200 K)Download as PowerPoint slide
Journal: Journal of Colloid and Interface Science - Volume 345, Issue 2, 15 May 2010, Pages 174–180