کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
611697 880682 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cationic agents for DNA compaction
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Cationic agents for DNA compaction
چکیده انگلیسی

Fluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CTAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy.

Fluorescence microscopy is used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. In the presence of proteins, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules. The maximum degree of compaction that is attained with iron(III) or CTAB is considerably smaller, and intermediate structures are visible even for high concentrations of these agents.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 323, Issue 1, 1 July 2008, Pages 75–83
نویسندگان
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