کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
611932 880688 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The N-terminal His-tag affects the enantioselectivity of staphylococcal lipases: A monolayer study
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
The N-terminal His-tag affects the enantioselectivity of staphylococcal lipases: A monolayer study
چکیده انگلیسی

In order to check the influence of the polyhistidine tag at the N-terminus of recombinant lipases, a comparative study on the interfacial properties of native and recombinant Staphylococcus simulans (SSL and rSSL) or Staphylococcus xylosus lipase (SXL and rSXL) was investigated using the monomolecular film technique. No phospholipase activity was detected with rSSL or rSXL when using different phospholipids spread as monomolecular films maintained at various surface pressures, suggesting that the His-tag in the N-terminus of the recombinant proteins, do not affect the substrate recognition. The critical surface pressure measured with monomolecular films of egg-PC was slightly lowered with the two recombinant proteins compared to the native SSL or SXL one. A kinetic study on the surface pressure dependency, stereoselectivity and regioselectivity of native and recombinant SSL or SXL was performed using three dicaprin isomers spread as monomolecular films at the air–water interface. Our results show clearly that the presence of polyhistidine tag at the N-terminus of SSL or SXL changes their stereo- and regioselectivity.

The N-terminal His-tag affects critically the regio- and the stereoselectivity of two recombinant staphylococcal lipases (Staphylococcus simulans lipase and Staphylococcus xylosus lipase).Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 313, Issue 1, 1 September 2007, Pages 261–267
نویسندگان
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