کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
612047 880690 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multi-color encoding of polystyrene microbeads with CdSe/ZnS quantum dots and its application in immunoassay
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Multi-color encoding of polystyrene microbeads with CdSe/ZnS quantum dots and its application in immunoassay
چکیده انگلیسی

Different diameter CdSe/ZnS semiconductor nanocrystals (average diameter: from ∼3.5 to ∼20 nm), quantum dots (QDs) were synthesized by changing the nucleation time, using organometallic reagents. These quantum dots possess narrow and symmetrical fluorescent emissions. The emission wavelengths of these composite dots span most of the visible spectrum from 500 through 700 nm. Furthermore, it is found that the quantum dots with an emission at ∼590 nm, tend to have a good quantum yield (such as Φ590=43.5%Φ590=43.5%). While the emission wavelength of prepared CdSe/ZnS QDs shifts toward blue or red from 590 nm, the quantum yield tends to decrease. After that, optical encoding of microbeads with these quantum dots was carried out, and the spectra of encoded beads were identified. The result indicates that, to identify the encoded beads with different emission wavelengths and emission intensities, it is needed to acquire and differentiate the spectra of beads. After immobilized with human IgG, the encoded beads were used to detect the corresponding antibody in solution. The result indicates that the encoded beads can detect the antibody signal effectively. And the effective detection range of the antibody is about 2–15 μM.

Multi-color encoding of microbeads with different quantum dots was carried out; the results indicate that, by using these encoded beads, the signal of the target molecule can be effectively identified.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 316, Issue 2, 15 December 2007, Pages 622–627
نویسندگان
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