کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
612431 1454614 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mitochondrial creatine kinase adsorption to biomimetic membranes: A Langmuir monolayer study
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Mitochondrial creatine kinase adsorption to biomimetic membranes: A Langmuir monolayer study
چکیده انگلیسی

Interaction of mitochondrial creatine kinase (mtCK) with either synthetic or natural zwitterionic or acidic phospholipids was monitored by surface pressure measurements. Injection of mtCK beneath a monolayer at very low surface pressure results in a large increase in the apparent area per lipid molecule reflecting the intrinsic surface activity of the protein. This effect is particularly pronounced with anionic phospholipid-containing films. Upon compression to high lateral pressure, the protein is squeezed out of the lipid monolayer. On the contrary, mtCK injected beneath a monolayer compressed at 30 mN/m, does not insert into the monolayer but is concentrated below the surface by anionic phospholipids as evidenced by the immediate and strong increase in the apparent molecular area occurring upon decompression. Below 8 mN/m the protein adsorbs to the interface and remains intercalated until the lateral pressure increases again. The critical pressure of insertion is higher for anionic lipid-containing monolayers than for films containing only zwitterionic phospholipids. In the former case it is markedly diminished by NaCl. The adsorption of mtCK depends on the percentage of negative charges carried by the monolayer and is reduced by increasing NaCl concentrations. However, the residual interaction existing in the absence of a global negative charge on the membrane may indicate that this interaction also involves a hydrophobic component.

Mitochondrial creatine kinase interacts with anionic phospholipid-containing monolayers in a ionic strength sensitive manner. An hydrophobic component is also involved in this interaction.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 310, Issue 2, 15 June 2007, Pages 436–445
نویسندگان
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