کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
612555 880701 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lysozyme binding onto cat-anionic vesicles
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Lysozyme binding onto cat-anionic vesicles
چکیده انگلیسی

Mixing aqueous sodium dodecylsulfate with cetyltrimethylammonium bromide solutions in mole ratios close to (1.7/1.0) allows the formation of cat-anionic vesicles with an excess of negative charges on the outer surface. The vesicular dispersions are mixed with lysozyme, and interact electrostatically with the positive charges on the protein, forming lipo-plexes. Dielectric relaxation, ζ-potential, and light scattering indicate the occurrence of interactions between vesicles and the protein. According to CD, the vesicle-adsorbed protein retains its native conformation. Binding and surface saturation, inferred by dielectric relaxation and ζ-potential, fulfil a charge neutralisation stoichiometry. Adsorbed lysozyme promotes the vesicle clustering and is concomitant with the lipo-plexes flocculation. Above the charge neutralisation threshold, lysozyme in excess remains dispersed in molecular form. Attempts were made to determine in what conditions protein release from the vesicles occurs. Accordingly, the full neutralisation of sodium dodecylsulfate in excess by cetyltrimethylammonium bromide ensures the lipo-plexes break-up, the precipitation of the mixed surfactants and the protein release in native form.

Cat-anionic vesicles made of SDS and CTAB interact with lysozyme. Interactions give rise to vesicle saturation. An investigation based on DLS, dielectric relaxation, CD, and ζ-potential indicates that lipo-plexes are being formed.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 304, Issue 2, 15 December 2006, Pages 342–347
نویسندگان
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