کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6288489 | 1616249 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Real-time PCR detection of Campylobacter spp.: A comparison to classic culturing and enrichment
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Real-time PCR detection of Campylobacter spp.: A comparison to classic culturing and enrichment Real-time PCR detection of Campylobacter spp.: A comparison to classic culturing and enrichment](/preview/png/6288489.png)
چکیده انگلیسی
The results indicate that the generic 16S campylobacter PCR detection is equally reliable but much faster (4 h instead of â¥2 days) than detection by means of culturing. Moreover, PCR detection targeting the hipO and the glyA gene provide the possibility of C. jejuni and C. coli species discrimination. The generic Campylobacter spp. PCR analysis also confirmed the high incidence of Campylobacter spp. in poultry samples (â¼90%) and the species specific PCR showed the simultaneous presence of C. jejuni and C. coli in â¼24% of the samples. Furthermore, the results from the three PCR analyses suggested the occurrence of alternative Campylobacter species in almost 10% of the samples. The campylobacter PCR detection methods reported here can replace traditional culturing because of being quicker and more reliable.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 51, October 2015, Pages 96-100
Journal: Food Microbiology - Volume 51, October 2015, Pages 96-100
نویسندگان
P. de Boer, H. Rahaoui, R.J. Leer, R.C. Montijn, J.M.B.M. van der Vossen,