کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6288600 | 1616254 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
lolB gene, a valid alternative for qPCR detection of Vibrio cholerae in food and environmental samples
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: lolB gene, a valid alternative for qPCR detection of Vibrio cholerae in food and environmental samples lolB gene, a valid alternative for qPCR detection of Vibrio cholerae in food and environmental samples](/preview/png/6288600.png)
چکیده انگلیسی
In recent years a new genetic target for Vibrio cholerae detection has been reported, but its application was limited to clinical samples. This target, lolB, has never been applied to either food or environmental samples. In the present study the development, as well as the evaluation and pre-validation, of a real-time PCR method based on lolB, is described. The method included a newly designed hydrolysis probe to enhance its specificity. After comparison against other molecular and traditional methods, similar results were obtained regarding relative sensitivity, relative specificity, relative accuracy, positive and negative predictive values and index kappa of concordance (all higher than 91%), as well as a very low limit of detection (2Â cfu/25Â g). Additionally, after the analysis of more than 160 different food and environmental samples, its applicability in the food industry was completely demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 46, April 2015, Pages 535-540
Journal: Food Microbiology - Volume 46, April 2015, Pages 535-540
نویسندگان
Alejandro Garrido-Maestu, MarÃa-José Chapela, Juan M. Vieites, Ana G. Cabado,